DNA-Catalyzed Amide Hydrolysis
نویسندگان
چکیده
منابع مشابه
DNA-Catalyzed Amide Hydrolysis.
DNA catalysts (deoxyribozymes) for a variety of reactions have been identified by in vitro selection. However, for certain reactions this identification has not been achieved. One important example is DNA-catalyzed amide hydrolysis, for which a previous selection experiment instead led to DNA-catalyzed DNA phosphodiester hydrolysis. Subsequent efforts in which the selection strategy deliberatel...
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Streptococcal proteinase was shown to belong to a group of enzymes such as trypsin, chymotrypsin, and subtilisin that exhibit a high ratio of esterase to peptidase activity. In this respect, streptococcal proteinase is different from other sulfhydryl enzymes, such as papain and ficin, and also different from pepsin. These enzymes hydrolyze a specific ester and amide substrate at comparable rate...
متن کاملDNA-catalyzed sequence-specific hydrolysis of DNA
Deoxyribozymes (DNA catalysts) have been reported for cleavage of RNA phosphodiester linkages, but cleaving peptide or DNA phosphodiester linkages is much more challenging. Using in vitro selection, here we identified deoxyribozymes that sequence-specifically hydrolyze DNA with multiple turnover and with a rate enhancement of 108 (possibly as high as 1014). The new DNA catalysts require both Mn...
متن کاملDNA-catalyzed hydrolysis of DNA phosphodiesters.
1. Shevach, E.M. Immunity 30, 636–645 (2009). 2. Yan, Z., Garg, S.K., Kipnis, J. & Banerjee, R. Nat. Chem. Biol. 5, 721–723 (2009). 3. Angelini, G. et al. Proc. Natl. Acad. Sci. USA 99, 1491–1496 (2002). 4. Castellani, P., Angelini, G., Delfino, L., Matucci, A. & Rubartelli, A. Eur. J. Immunol. 38, 2419–2425 (2008). 5. Kazama, H. et al. Immunity 29, 21–32 (2008). 6. Lim, S.Y., Raftery, M.J., Go...
متن کاملDNA-catalyzed hydrolysis of esters and aromatic amides.
We previously reported that DNA catalysts (deoxyribozymes) can hydrolyze DNA phosphodiester linkages, but DNA-catalyzed amide bond hydrolysis has been elusive. Here we used in vitro selection to identify DNA catalysts that hydrolyze ester linkages as well as DNA catalysts that hydrolyze aromatic amides, for which the leaving group is an aniline moiety. The aromatic amide-hydrolyzing deoxyribozy...
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ژورنال
عنوان ژورنال: Journal of the American Chemical Society
سال: 2016
ISSN: 0002-7863,1520-5126
DOI: 10.1021/jacs.5b12647